Bos taurus Raw sequence reads - Retrospective Model. Bos taurus breed:Nelore

Single-cell RNA-Seq from Metaphase II oocyte and oocyte biopsy were sequenced. We first compared the oocyte biopsy with the entirely respective oocyte usina g single-cell RNA-Seq approach The second experiment compared oocyte biopsies after chemical activation followed by embryo culture. In this experiment, two groups were compared using biopsies. Biopsies from non-competent oocytes (arrested on day 3) and competent ones (developed until day 7); From the same follicle samples, we also performed Sc-RNA-Seq in granulosa collected before in vitro maturation. The cumulus cells after in vitro maturation were performed using pooled samples We also performed WGBS from the single polar body collected from the same oocytes utilized in the RNA-Seq experiment. These were the first polar body (MII phase)