Microbial thermal plasticity in composting of lignocellulosic waste: Dataset and Supplementary Material

This study was built upon a large collection of bacteria, actinobacteria and fungi (1380 strains) previously isolated through an intensive sampling of a composting process and identified by molecular methods. This collection of strains supposed the entire cultivable microbiota associated to the composting process investigated. Since a significant number of strains were isolated many times, regardless of the temperature inside the composting piles (which ranged from 21.1 to 68.3 ºC), the starting hypothesis raised in this work was that a high thermotolerance can be found in the microbiota associated with the composting process.
All strains were grown under laboratory conditions at 20, 30, 40, 50 and 60 ºC and growth at optimal temperature was recorded for each strain. In addition, the extent of growth inhibition at non-optimal temperatures was also measured.
Results obtained showed a high thermal plasticity (thermotolerance) in the composting microbiome, since 90% of the strains grew at all tested temperatures. Only 9% and 1% of strains were classified as strictly mesophilic or strictly thermophilic, respectively. According to these results, the traditional conception of microbial groups succession throughout a composting process would need to be reassessed.
Files in this dataset show the evolution of control parameters throughout the studied composting process and data from the experiments of quantification of growth at the assayed temperatures for the 1380 strains, as well as their taxonomic position.

本研究基于一大批细菌、放线菌和真菌（共1380株）的丰富集合，这些菌株通过在堆肥过程中进行密集采样并采用分子方法进行鉴定而获得。该菌株集合假定了与所研究的堆肥过程相关的全部可培养微生物群。鉴于大量菌株在不同堆肥堆体内部温度（21.1至68.3 ºC不等）下多次分离，本研究提出的起始假设是，堆肥过程相关的微生物群中可能存在高度的热耐受性。所有菌株均在20、30、40、50和60 ºC的实验室条件下培养，并记录了每株菌株在最佳温度下的生长情况。此外，还测量了非最佳温度下生长抑制的程度。研究结果揭示了堆肥微生物群具有高度的热塑性（热耐受性），因为90%的菌株在所有测试的温度下均能生长。仅有9%和1%的菌株分别被归类为严格嗜温菌和严格嗜热菌。据此，对堆肥过程中微生物群组演替的传统观念将需要重新评估。该数据集中的文件展示了控制参数在整个堆肥过程中的演变，以及针对1380株菌株在测试温度下的生长量度实验数据及其分类学位置。