Growing Malaria Parasites at a Critical Shaking Speed Mimicking Physiological Flow Reveals New Phenotypes for Invasion Ligands, Supporting Information

This repository contains the dataset and analysis scripts associated with the upcoming publication titled Growing malaria parasites at a critical shaking speed that mimics physiological flow conditions reveals new phenotypes for EBA and RH invasion ligands. The repository includes a comprehensive collection of data and scripts used to generate all the plots, along with videos showing how the red blood cells behave in culture media for different shaking speeds in the different shaking vessels. The growth assay data used for this experiment was collected in four batches, labelled GA1 to 4: GA1: compares the different knockout lines. GA2: compares different hematocrits. GA3: compares different growth vessels. GA4: contains more repeats of lines from GA1. This repository offers all necessary resources to replicate the findings, including the complete codebase, raw data, and graphical representations of results. Researchers are encouraged to explore the included notebooks and datasets for detailed insights. Movies S1 to S4 show a top and side view of each type of plate/plask used on an orbital shaker as the shaking speed is increased.本仓库包含与即将发表的论文相关的数据集与分析脚本，该论文标题为《通过临界振荡转速模拟生理流动条件培养疟原虫：为EBA（Erythrocyte Binding Antigens）与RH（Reticulocyte Binding Homologs）入侵配体揭示新表型》。 本仓库收录了用于生成全部绘图的完整数据集与脚本集合，同时包含展示不同振荡培养容器内，不同振荡转速下红细胞在培养基中的行为表现的视频素材。 本实验所用的生长测定数据分为四批，编号为GA1至GA4： GA1：对比不同基因敲除株系 GA2：对比不同血细胞比容 GA3：对比不同培养容器 GA4：包含GA1中所用株系的更多重复实验数据 本仓库提供了重复该研究结果所需的全部资源，包括完整代码库、原始数据以及结果的可视化图表。我们鼓励研究人员浏览仓库内的交互式笔记本与数据集，以获取详细的研究见解。 视频S1至S4展示了随着振荡转速升高，轨道式摇床上所用的各类培养板/培养槽的顶视与侧视画面。