Kolmancic-Zivin-Zorovic_E2_L-dopa_data2022.sav

The data are the results of behavioural testing and immunohistochemistry from a study on the effects of estrogen on the development of levodopa-induced dyskinesias in a hemiparkinsonian postmenopausal rat model. The animal study protocol was approved by the Republic of Slovenia's Administration for Food Safety, Veterinary Sector and Plant Protection (ref. no. U34401-7/2017/9). The rats were overiectomized at the start of the experiment. Two weeks following the 6-OHDA lesion of dopaminergic neurons in the right hemisphere and implantation of either empty (control) or estradiol implants, the animals started recieiving daily injections of either levodopa or saline (control). Animals were divided into four tratment groups: animals with estradiol implants and daily levodopa injections, animals with empty implants and daily levodopa injections, animals with estradiol implants and daily injections of saline and animals with empty implants and daily saline injections. Behavioural testing included tests to establish the level of levodopa-induced dyskinesias (LIDs), rotation tests (RT) and the forepaw adjusting step (FAS) test to assess the motor performance. Animal body weight was followed throughout the experiment and serum levels of estradiol were measured after euthanasia. Immunohistochemistry with anti-TH primary antibody was performed on frozen brain sections to establish the extent of the 6-OHDA lesion. We also evaluated the changes in expression of FosB protein in animals from different treatments.<br>本数据集为一项探究雌激素对偏帕金森病绝经后大鼠模型中左旋多巴诱发异动症（levodopa-induced dyskinesias, LIDs）影响的研究的行为学检测与免疫组化实验结果。该动物实验方案已获斯洛文尼亚共和国食品安全、兽医与植物保护管理局批准（批准编号：U34401-7/2017/9）。实验伊始对大鼠实施卵巢切除术。在对大鼠右侧大脑半球多巴胺能神经元进行6-羟基多巴胺（6-OHDA）损毁，并植入空白（对照组）或雌二醇植入物两周后，所有大鼠开始每日接受左旋多巴或生理盐水（对照组）注射。实验共分为四组：植入雌二醇并每日注射左旋多巴组、植入空白植入物并每日注射左旋多巴组、植入雌二醇并每日注射生理盐水组，以及植入空白植入物并每日注射生理盐水组。行为学检测包含左旋多巴诱发异动症严重程度评估、旋转实验（rotation tests, RT）与前爪调整步幅（forepaw adjusting step, FAS）实验，用以评价大鼠运动功能。实验全程监测大鼠体重，并在安乐死后采集血清样本检测雌二醇水平。采用抗酪氨酸羟化酶（tyrosine hydroxylase, TH）一抗对冰冻脑切片开展免疫组化检测，以明确6-OHDA损毁范围。本研究同时分析了不同处理组大鼠体内FosB蛋白的表达变化。