Additional file 2: of Pregnancy and estrogen enhance neural progenitor-cell proliferation in the vomeronasal sensory epithelium

Excel workbook containing the mapping statistics of the RNAseq data for each sample, along with the accession numbers for the raw data; the normalized counts for the whole transcriptome; the differential expression analysis between the pregnant and control samples; the gene ontology categories significantly overrepresented in the differentially expressed genes; the normalized counts for the VR genes, accounting for total VSN number; and the differential expression analysis on the VR genes only, after normalization for VSN number, between the pregnant and control samples. The column ‘length’ corresponds to the total exonic bases in all transcripts from the gene. For the differential expression sheets, the columns contain the following data: ‘baseMean’, corresponds to the mean normalized expression value for the gene across all samples; ‘log2FoldChange’, is the fold change between the pregnant and control samples, log2 transformed; ‘lfcSE’, corresponds to the standard error associated with the fold change estimation; ‘stat’, is the Wald statistic; ‘pvalue’ is the P value of the test; and ‘padj’ is the P value after adjusting for multiple testing (Benjamini-Hochberg). Genes that have both their ‘pvalue’ and ‘padj’ set to NA contain outliers; genes with only their ‘padj’ set to NA were filtered prior to the test because their normalized counts were too low. (XLSX 11150 kb)

本Excel工作簿包含以下内容：各样本RNA测序（RNAseq）数据的比对统计信息、原始数据的登录号、全转录组标准化计数结果、妊娠组与对照组的差异表达分析结果、差异表达基因中显著富集的基因本体（Gene Ontology）分类、校正总VSN数后的VR基因标准化计数结果，以及仅针对VR基因、在完成VSN数标准化校正后开展的妊娠组与对照组差异表达分析。其中“length”列对应基因所有转录本的外显子总碱基数。针对差异表达分析工作表，各列含义如下：“baseMean”为所有样本中该基因的平均标准化表达值；“log2FoldChange”为妊娠组与对照组的表达倍数变化经log₂转换后的值；“lfcSE”为倍数变化估计值对应的标准误；“stat”为Wald统计量；“pvalue”为检验的原始P值；“padj”为经多重检验校正（Benjamini-Hochberg）后的校正P值。若基因的“pvalue”与“padj”均为NA，则该基因存在异常值；若仅“padj”为NA，则该基因因标准化计数过低，已在检验前被过滤。本文件格式为XLSX，大小为11150 KB。