The FPKM analysis of 71 UGTs from C. orchioides.

Orcinol glucoside (OG), mainly found in the rhizome of the traditional Chinese herb Curculigo orchioides Gaertn, is noted for its antidepressant effects. In this study, an efficient screening pipeline was established for identifying the highly active orcinol synthase (ORS) and UDP-dependent glycosyltransferase (UGT) involved in the biosynthesis of OG by combining transcriptome analysis, structure-based virtual screening, and in vitro enzyme activity assays. By enhancing the downstream pathway, metabolic engineering and fermentation optimization, the OG production in Yarrowia lipolytica was improved 100-fold, resulting in a final yield of 43.46 g/L (0.84 g/g DCW), which is almost 6,400-fold higher than the extraction yield from C. orchioides roots. This study provides a reference for rapid identification of functional genes and high-yield production of natural products.地衣二醇葡萄糖苷（Orcinol glucoside，OG）主要分布于传统中药仙茅（Curculigo orchioides Gaertn）的根茎中，其抗抑郁活性已得到广泛关注。本研究结合转录组分析、基于结构的虚拟筛选与体外酶活测定技术，搭建了一套高效筛选流程，用于鉴定参与OG生物合成的高活性地衣二醇合酶（orcinol synthase，ORS）与UDP依赖型糖基转移酶（UDP-dependent glycosyltransferase，UGT）。通过强化下游代谢通路、实施代谢工程改造与发酵工艺优化，解脂耶氏酵母（Yarrowia lipolytica）中的OG产量提升了100倍，最终产量达到43.46 g/L（0.84 g/g 细胞干重（DCW）），较从仙茅根中提取的产量高出近6400倍。本研究为功能基因的快速鉴定及天然产物的高产制备提供了可靠参考范式。