Single-cell RNA sequencing data of stem cell-derived microfluidic human embryoids, NODAL-KO human embryoids and chimpanzee embryoids

Transcriptome at the single-cell level of a human embryoid model, which recapitulates aspects of lineage diversification and three-dimensional tissue architecture of the human embryo from the implantation to the onset of gastrulation, was profiled at different time points. Similarly, chimpanzee embryoids were generated and profiled to reveal transcriptome dynamics during the early post-implantation chimpanzee development. Our comparative transcriptome analyses reveal a critical role of NODAL signaling in human mesoderm specification, which is functionally validated by generating embryoids with NODAL-knockout (KO) hPSCs. We applied single-cell mRNA sequencing technique to examine the transcriptomes of microfluidic embryoids. The .Rd files can be loaded into RStudio using "load ("file_name.Rd")", and analyzed using the Seurat R package (https://satijalab.org/seurat/). In case of difficulties accessing the data, please contact Dr. Yi Zheng ([e-mail])