Summary of Key Findings.

The combined use of Zingiber officinale (ginger) and Citrus limon (lemon) has been traditionally recognized for its therapeutic potential, particularly in promoting cardiovascular health. However, the synergistic effects of these botanicals on hyperlipidemia and oxidative stress, two major contributors to cardiovascular disease, remain insufficiently characterized. This study employed an integrative approach involving in vivo, in vitro, and in silico methodologies to assess the hypolipidemic and antioxidant properties of ginger and lemon juices. In vivo experiments were conducted using a murine model of Triton WR-1339-induced hyperlipidemia. Antioxidant activity was evaluated through DPPH and ABTS assays, while molecular docking simulations were performed to assess interactions with HMG-CoA reductase, a key enzyme in cholesterol metabolism. Phytochemical profiling revealed high levels of bioactive compounds, with ginger juice containing 6-gingerol (15.54% peak area), and lemon juice rich in hesperidin (13.85%) and rutin (5.57%). These constituents likely contributed to the observed pharmacological effects. The combined formulation led to marked improvements in lipid parameters, including reductions in total cholesterol (−54.3%), triglycerides (−49.8%), and LDL-C (−58.1%), alongside an increase in HDL-C (+47.6%) compared to hyperlipidemic controls (p Zingiber officinale and Citrus limon juices exhibited potent lipid-lowering and antioxidant activities, outperforming the effects observed with individual extracts. These findings underscore their potential as natural therapeutic agents for the management of hyperlipidemia and oxidative stress. Further clinical investigations are warranted to validate these preclinical results and to explore their future application in the development of nutraceuticals or functional foods.生姜(Zingiber officinale)与柠檬(Citrus limon)的联合使用，在传统认知中便具备治疗潜力，尤其在促进心血管健康方面。然而，这两种植物成分在高脂血症与氧化应激——两大心血管疾病主要致病因素——中的协同效应，仍未得到充分阐明。本研究采用整合研究方法，结合体内(in vivo)、体外(in vitro)与计算机模拟(in silico)三种手段，评估生姜与柠檬汁的降血脂与抗氧化特性。体内实验采用Triton WR-1339诱导的高脂血症小鼠模型开展；抗氧化活性通过DPPH与ABTS实验进行评价；同时开展分子对接模拟，以探究其与胆固醇代谢关键酶——羟甲基戊二酰辅酶A还原酶(HMG-CoA reductase)——的相互作用。植物化学组分分析结果显示，二者均含有高水平生物活性成分：生姜汁中6-姜酚(6-gingerol)的峰面积占比达15.54%；柠檬汁则富含橙皮苷(hesperidin，13.85%)与芦丁(rutin，5.57%)。上述成分或为其发挥观测到的药理作用的关键物质基础。与高脂血症模型对照组相比，复合制剂可显著改善血脂参数：总胆固醇降低54.3%、甘油三酯降低49.8%、低密度脂蛋白胆固醇(LDL-C)降低58.1%，同时高密度脂蛋白胆固醇(HDL-C)升高47.6%（p值差异具有统计学意义）；生姜与柠檬汁单独应用时亦展现出强效的降血脂与抗氧化活性，且效果优于单一提取物组。本研究结果证实，二者联合应用具备作为治疗高脂血症与氧化应激的天然治疗制剂的潜力。未来需开展进一步临床研究，以验证上述临床前研究结果，并探索其在营养补充剂或功能性食品开发中的应用前景。