Antioxidant activitiy of Lactobacillus plantarum , Lactobacillus sake and Lactobacillus curvatus strains isolated from fermented Turkish Sucuk

Abstract In recent years, it is very important to find more safe and natural antioxidants than biological sources to replace synthetic antioxidants. The antioxidant properties of 22 lactic acid bacteria were investigated in vitro in the present study. The conducted in vitro antioxidant assays included scavenging the 2,2-diphenyl-1-picrylhydrazil (DPPH) free radical, metal (Fe+2) ion chelation, hydroxyl radical and superoxide radical scavenging properties, and anti-lipid peroxidation. Analysis of DPPH free radical scavenging property of microorganisms demonstrated that Lactobacillus plantarum IH14L (90.34 ± 0.40%) strain exhibited the highest activity. The highest Fe+2 chelation activity was observed with Lactobacillus curvatus GH5L (75.98 ± 5.35%), while the lowest chelation activity was observed with L. plantarum IH14L (19.80 ± 0,05). The highest and lowest hydroxyl radical scavenging strains were L. plantarum IH16L (82.25 ± 1.60%) and L. plantarum IH26L (35.60 ± 4.50) strains, respectively. Comparison of superoxide radical scavenging activities of the microorganisms demonstrated that these activities ranged between 21.63 ± 1.32% and 7.22 ± 0.04%. A positive correlation was identified between the hydroxyl radical scavenging activity of the microorganisms and the anti-lipid peroxidation property. It was found that they had the potential for use in nutritional and probiotic applications as protective and natural antioxidants.

摘要 近年来，筛选安全性与天然性更优的抗氧化剂以替代合成抗氧化剂，已成为领域内的重要研究议题。本研究通过体外实验评估了22株乳酸菌的抗氧化活性。所开展的体外抗氧化检测包括2,2-二苯基-1-苦基肼自由基（DPPH）清除能力、Fe²+金属离子螯合能力、羟基自由基与超氧阴离子自由基清除能力，以及抗脂质过氧化活性。对供试菌株DPPH自由基清除活性的分析显示，植物乳杆菌（Lactobacillus plantarum）IH14L菌株的清除活性最高，达90.34 ± 0.40%。弯曲乳杆菌（Lactobacillus curvatus）GH5L菌株的Fe²+螯合活性最强，为75.98 ± 5.35%，而植物乳杆菌IH14L的螯合活性最低，仅为19.80 ± 0.05。羟基自由基清除能力最强和最弱的菌株分别为植物乳杆菌IH16L（82.25 ± 1.60%）与植物乳杆菌IH26L（35.60 ± 4.50%）。对供试菌株超氧阴离子自由基清除活性的比较显示，其活性范围为7.22 ± 0.04%至21.63 ± 1.32%。研究发现，供试菌株的羟基自由基清除活性与抗脂质过氧化活性呈正相关。综上，这些乳酸菌具备作为保护性天然抗氧化剂应用于营养与益生菌相关领域的潜力。