PacBio full-length 16S rRNA raw sequencing data from the study: The effect of daily oral probiotic and postbiotic supplementation on the canine skin microbiota

This dataset contains raw PacBio HiFi long-read fastq files of full-length 16S rRNA gene sequences derived from skin swab samples (axillae and inguinal regions) of 12 healthy golden retrievers. Samples were collected as part of a longitudinal study at Day 0, Day 30, and Day 90 of oral daily probiotic/postbiotic supplementation (ODPPS). This study investigates the impact of oral biotic ODPPS on skin microbiota composition using high-resolution long-read sequencing. DNA was extracted and sequenced using the PacBio Sequel platform to generate long-read 16S rRNA profiles for comprehensive microbial community analysis. This data supports the findings presented in the manuscript titled “The effect of daily oral probiotic and postbiotic supplementation on the canine skin microbiota: insights from culture-dependent and long-read 16S rRNA gene sequencing methods”, recently accepted in Veterinary Dermatology, Manuscript ID VDE-2024-149.R2. The dataset is intended for reuse in comparative microbiome studies and method development in veterinary microbiology and skin health.本数据集包含来自12只健康金毛寻回犬皮肤拭子样本（腋窝与腹股沟区域）的全长16S核糖体RNA（16S rRNA）基因序列的PacBio HiFi长读长fastq格式原始文件。上述样本采集自一项纵向研究，采集时点为受试犬每日口服益生菌/益生元补充剂（oral daily probiotic/postbiotic supplementation，缩写ODPPS）干预的第0天、第30天及第90天。本研究借助高分辨率长读长测序技术，旨在探究口服生物制剂ODPPS对犬皮肤微生物群组成的影响。 研究采用PacBio Sequel测序平台对提取的DNA进行测序，以生成长读长16S rRNA谱，用于全面的微生物群落分析。 本数据集支撑了近日被《兽医皮肤病学》(Veterinary Dermatology)录用的题为"每日口服益生菌与益生元补充剂对犬皮肤微生物群的影响：基于培养依赖法与长读长16S rRNA基因测序技术的解析"的论文（稿件编号VDE-2024-149.R2）的研究结论。本数据集可用于兽医微生物学与皮肤健康领域的比较微生物组研究及相关方法开发的再利用。