CIL:46280. In Cell Image Library

Plate 25707 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.

Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).

This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

编号25707的微孔板——表型谱分析（Phenotypic Profiling）旨在通过多参数、基于特征的分析手段，对每个样本的细胞表型进行全面总结，使得谱图间的相似性能够反映样本间的生物学相似性。该图像集为基于图像的谱分析方法的性能验证提供了基准平台，可用于评估其区分生物活性小分子化合物作用效果的能力。

图像说明
本图像集的成像对象为经1600种已知生物活性化合物分别处理的U2OS细胞（人骨肉瘤细胞系），通过六种标记物对七种细胞器进行染色标记，该实验为「细胞绘画（cell-painting）」检测实验。

本次预实验共包含20块微孔板。每块微孔板设有384个孔位，每个孔位采集9个成像视场，总计69120个成像视场。每个成像视场通过5个检测通道（对应不同检测波长）完成成像，且每个通道的图像均以独立的灰度图像文件存储，因此该数据集共包含345600张16位TIFF格式的图像文件。