Supplementary Datasets for the publication "Rousettus aegyptiacus Fruit Bats Do Not Support Productive Replication of Cedar Virus upon Experimental Challenge"

Cedar henipavirus (CedV), which was isolated from the urine of pteropodid bats in Australia, belongs to the genus Henipavirus in the family of Paramyxoviridae. It is closely related to the Hendra virus (HeV) and Nipah virus (NiV), which have been classified at the highest biosafety level (BSL4) due to their high pathogenicity for humans. Meanwhile, CedV is apathogenic for humans and animals. As such, it is often used as a model virus for the highly pathogenic henipaviruses HeV and NiV. In this study, we challenged eight Rousettus aegyptiacus fruit bats of different age groups with CedV in order to assess their age-dependent susceptibility to a CedV infection. Upon intranasal inoculation, none of the animals developed clinical signs, and only trace amounts of viral RNA were detectable at 2 days post-inoculation in the upper respiratory tract and the kidney as well as in oral and anal swab samples. Continuous monitoring of the body temperature and locomotion activity of four animals, however, indicated minor alterations in the challenged animals, which would have remained unnoticed otherwise.Cedar亨尼帕病毒（Cedar henipavirus, CedV）分离自澳大利亚狐蝠科蝙蝠的尿液，隶属于副黏病毒科（Paramyxoviridae）亨尼帕病毒属。其与亨德拉病毒（Hendra virus, HeV）、尼帕病毒（Nipah virus, NiV）亲缘关系密切，后两者因对人类具有高致病性，被划分为生物安全四级（BSL4）。而CedV对人类与动物均无致病性，因此常被用作高致病性亨尼帕病毒HeV与NiV的模型病毒。本研究选取8只不同年龄段的埃及果蝠（Rousettus aegyptiacus），通过鼻内接种方式感染CedV，以评估其对CedV感染的年龄依赖性易感性。结果显示，所有受试动物均未出现临床症状，仅在接种后2天的上呼吸道、肾脏以及口腔与肛拭子样本中可检测到微量病毒RNA。不过，对其中4只动物的体温与运动活动进行持续监测后发现，感染组动物存在轻微的生理变化，若未开展该项监测，此类变化将难以被察觉。