Abundance of mesozooplankton in the western part of the Black Sea in spring 1997 during the National Monitoring Programme

The "15BO1997001" dataset is based on samples collected in the spring of 1997. The whole dataset is composed of 66 samples (from 27 stations of National Monitoring Sampling Grid) with data of zooplankton species composition, abundance and biomass. Samples were collected in discrete layers 0-10, 0-20, 0-50, 10-25, 25-50, 50-100 and from bottom up to the surface at depths depending on water column stratification and the thermocline depth.
Zooplankton samples were collected with vertical closing Juday net,diameter - 36cm, mesh size 150 µm. Tows were performed from surface down to bottom meters depths in discrete layers. Samples were preserved by a 4% formaldehyde sea water buffered solution. Sampling volume was estimated by multiplying the mouth area with the wire length.
Mesozooplankton abundance: The collected material was analysed using the method of Domov (1959). Samples were brought to volume of 25-30 ml depending upon zooplankton density and mixed intensively until all organisms were distributed randomly in the sample volume. After that 5 ml of sample was taken and poured in the counting chamber which is a rectangle form for taxomomic identification and count. Large (> 1 mm body length) and not abundant species were calculated in whole sample. Counting and measuring of organisms were made in the Dimov chamber under the stereomicroscope to the lowest taxon possible. Taxonomic identification was done at the Institute of Oceanology by Lyudmila Kamburska using the relevant taxonomic literature (Mordukhay-Boltovskoy, F.D. (Ed.). 1968, 1969,1972).
Taxon-specific abundance: The collected material was analysed using the method of Domov (1959). Samples were brought to volume of 25-30 ml depending upon zooplankton density and mixed intensively until all organisms were distributed randomly in the sample volume. After that 5 ml of sample was taken and poured in the counting chamber which is a rectangle form for taxomomic identification and count. Copepods and Cladoceras were identified and enumerated; the other mesozooplankters were identified and enumerated at higher taxonomic level (commonly named as mesozooplankton groups). Large (> 1 mm body length) and not abundant species were calculated in whole sample. Counting and measuring of organisms were made in the Dimov chamber under the stereomicroscope to the lowest taxon possible. Taxonomic identification was done at the Institute of Oceanology by Lyudmila Kamburska using the relevant taxonomic literature (Mordukhay-Boltovskoy, F.D. (Ed.). 1968, 1969,1972).

"15BO1997001"数据集基于1997年春季采集的样本。完整数据集包含66份样本（取自国家监测采样网格的27个站位），涵盖浮游动物物种组成、丰度与生物量数据。采样分层设置为0-10、0-20、0-50、10-25、25-50、50-100米，以及依据水体层化结构和温跃层深度确定的从水底至表层的分层采样。

浮游动物样本采用垂直封闭型Juday网（Juday net）采集，该网直径36cm，网目尺寸150μm。拖网作业按离散分层从表层下至指定底层水深进行。样本采用4%甲醛海水缓冲液进行固定保存。采样体积通过网口面积与拖曳绳长度的乘积估算。

中型浮游动物丰度分析：采集的样品采用Domov（1959）方法开展分析。根据浮游动物密度将样品定容至25-30ml，充分混匀直至所有生物在样液中随机均匀分布。随后取5ml样品注入矩形计数框，用于分类鉴定与个体计数。体长大于1mm且丰度较低的物种以全样进行统计。在体视显微镜下使用迪莫夫计数框（Dimov chamber）对生物个体进行计数与形态测量，尽可能将物种鉴定至最低分类阶元。物种分类鉴定由Lyudmila Kamburska在海洋学研究所完成，参考相关分类学文献（Mordukhay-Boltovskoy, F.D. (Ed.). 1968, 1969,1972）。

分类单元特异性丰度分析：采集的样品仍采用Domov（1959）方法开展分析。根据浮游动物密度将样品定容至25-30ml，充分混匀直至所有生物在样液中随机均匀分布。随后取5ml样品注入矩形计数框，用于分类鉴定与个体计数。其中桡足类（Copepods）与枝角类（Cladoceras）需鉴定至物种并完成计数；其余中型浮游动物则按较高分类阶元（通常称为中型浮游动物类群）进行鉴定与计数。体长大于1mm且丰度较低的物种以全样进行统计。在体视显微镜下使用迪莫夫计数框（Dimov chamber）对生物个体进行计数与形态测量，尽可能将物种鉴定至最低分类阶元。物种分类鉴定由Lyudmila Kamburska在海洋学研究所完成，参考相关分类学文献（Mordukhay-Boltovskoy, F.D. (Ed.). 1968, 1969,1972）。