In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes.

Abstract: INTRODUCTION: Dengue fever is a viral disease transmitted by the Aedes aegypti Linn. (1792) (Diptera: Culicidae) mosquito, which is endemic in several regions of Brazil. Alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower environmental contamination levels and less likelihood of resistant populations. Thus, in this study, the ability of botanical insecticide formulations to inhibit the activity of the liver enzymes serum cholinesterase and malate dehydrogenase was evaluated. METHODS: Inhibition profiles were assessed using in vitro assays for cholinesterase and malate dehydrogenase activity and quantitated by ultraviolet-visible spectroscopy at 410nm to 340nm. RESULTS Insecticide products formulated from cashew nutshell liquid [A] and ricinoleic acid [B] showed cholinesterase activity levels of 6.26IU/mL and 6.61IU/mL, respectively, while the control level for cholinesterase was 5-12IU/mL. The products did not affect the level of 0.44IU/mL established for malate dehydrogenase, as the levels produced by [A] and [B] were 0.43IU/mL and 0.45IU/mL, respectively. CONCLUSIONS Our findings show that in vitro testing of the formulated products at concentrations lethal to A. aegypti did not affect the activity of cholinesterase and malate dehydrogenase, indicating the safety of these products.摘要：引言：登革热是由埃及伊蚊（Aedes aegypti Linn. 1792，双翅目：蚊科）传播的病毒性疾病，在巴西多个地区呈地方性流行。防治该媒介生物的替代方法包括植物源杀虫剂，其具备环境污染物水平更低、抗药性种群产生概率更小等优势。据此，本研究评估了植物源杀虫剂制剂对血清胆碱酯酶与苹果酸脱氢酶这两种肝脏酶活性的抑制作用。 方法：通过体外胆碱酯酶与苹果酸脱氢酶活性实验对抑制谱进行评估，并采用紫外-可见分光光度法在410nm至340nm波长下完成定量检测。 结果：由腰果壳液[A]与蓖麻油酸[B]配制的杀虫剂产品，其胆碱酯酶活性分别为6.26IU/mL和6.61IU/mL，而胆碱酯酶的对照活性水平为5~12IU/mL。上述产品对苹果酸脱氢酶0.44IU/mL的标准活性水平无影响，[A]和[B]对应的检测值分别为0.43IU/mL和0.45IU/mL。 结论：本研究结果表明，在达到埃及伊蚊致死浓度的条件下，所测试的制剂对胆碱酯酶与苹果酸脱氢酶活性无影响，提示上述产品具备安全性。