Mammary single-cell RNA-seq analysis and prostate cancer survival as a function of H2AFJ expression for the paper entitled: The histone variant H2A.J is enriched in luminal epithelial cells

H2A.J is a poorly studied mammalian-specific variant of histone H2A. We used immunohistochemistry to study its localization in various human and mouse tissues. H2A.J showed cell-type specific expression with a striking enrichment in luminal epithelial cells of multiple glands including those of breast, prostate, pancreas, thyroid, stomach, and salivary glands. H2A.J was also highly expressed in many carcinoma cell lines and in particular, those derived from luminal breast and prostate cancer. H2A.J thus appears to be a novel marker for luminal epithelial cancers. Knocking-out the H2AFJ gene in T47D luminal breast cancer cells reduced the expression of several estrogen-responsive genes which may explain its putative tumorigenic role in luminal-B breast cancer.H2A.J是一种研究尚不充分的哺乳动物特异性组蛋白H2A变体。我们采用免疫组织化学技术，探究其在多种人类及小鼠组织中的定位分布。H2A.J呈现细胞类型特异性表达模式，在乳腺、前列腺、胰腺、甲状腺、胃及唾液腺等多种腺体的腔上皮细胞中显著富集。H2A.J在多种癌细胞系中亦呈高表达状态，尤其在源自腔型乳腺癌与前列腺癌的细胞系中表达水平尤为突出。据此，H2A.J可作为腔上皮细胞源性癌症的新型分子标志物。在T47D腔型乳腺癌细胞中敲除H2AFJ基因，可下调多种雌激素应答基因的表达，这或可解释其在腔型B乳腺癌中潜在的致瘤作用。