Italian CryoEM Bag (ICB)

This BAG application builds on the development of single particle cryo-EM in the Italian Structural Biology community, as in Italy there is no top-end instrument available. The groups in this application have acquired know-how in cryo.EM through international collaborations or by acquiring medium-level in-house equipment. Access to the CM01 beamline at the ESRF has been instrumental for the achievement of high-profile scientific results and publications in top journals. Presently three screening microscopes are available in Italy, these instruments will boost sample production and the number of applications to CM01. Given these premises a BAG from Italian institutions could ensure timely data collection, nurturing the expansion of the field in Italy and a high-level scientific production.

本BAG申请立足于意大利结构生物学界单粒子冷冻电镜（single particle cryo-EM）的发展现状：目前意大利尚无高端冷冻电镜仪器可用。本申请涉及的科研团队通过国际合作或购置中端自主实验设备，掌握了冷冻电镜相关技术诀窍。借助欧洲同步辐射光源（European Synchrotron Radiation Facility, ESRF）的CM01光束线，团队已取得多项高影响力科研成果，并在顶级学术期刊发表论文。目前意大利已配备三台筛选用显微镜，此类设备将有效提升样品制备产能，并增加CM01光束线的申请量。基于上述背景，来自意大利科研机构的BAG申请可保障数据采集的时效性，助力该领域在意大利的发展，并产出高水平科研成果。