S1 File - Siaα2-3Galβ1- Receptor Genetic Variants Are Associated with Influenza A(H1N1)pdm09 Severity

Table A in S1 File. In silico functional analysis results for rs113350588 and rs1048479 and variants in Linkage disequilibrium. *Refers to LD between the rs2142306, rs2736865 and rs1048479 polymorphism for 1000 Genome project. Table B in S1 File. Frequency of the ST3GAL1 gene haplotypes observed in patients infected with Influenza A(H1N1)pdm09. Table C in S1 File. ST3GAL1 gene diplotypes frequencies observed in patients infected with Influenza A(H1N1)pdm09. Table D in S1 File. Sequence of the primers used for PCR amplification and nucleotide sequencing of the ST3GAL1 gene. Table E in S1 File. Protocol for PCR amplification. aMixture of deoxyribonucleotide triphosphates: dATP, dCTP, dGTP, and dTTP. bA total of 35 cycles were performed for reactions with the exception of exon 8. cAnneling: 65°C 2 cycles; 64°C 10 cycles; 62°C 10 cycles, and 60°C 15 cycles. Table F in S1 File. Protocol for sequencing. aPerformed with 35 cycles. Table G in S1 File. Allele and genotype frequency of the ST3GAL1 gene polymorphisms in 68 patients infected with Influenza A(H1N1)pdm09. (DOCX)