S1 File - Siaα2-3Galβ1- Receptor Genetic Variants Are Associated with Influenza A(H1N1)pdm09 Severity

Table A in S1 File. In silico functional analysis results for rs113350588 and rs1048479 and variants in Linkage disequilibrium. *Refers to LD between the rs2142306, rs2736865 and rs1048479 polymorphism for 1000 Genome project. Table B in S1 File. Frequency of the ST3GAL1 gene haplotypes observed in patients infected with Influenza A(H1N1)pdm09. Table C in S1 File. ST3GAL1 gene diplotypes frequencies observed in patients infected with Influenza A(H1N1)pdm09. Table D in S1 File. Sequence of the primers used for PCR amplification and nucleotide sequencing of the ST3GAL1 gene. Table E in S1 File. Protocol for PCR amplification. aMixture of deoxyribonucleotide triphosphates: dATP, dCTP, dGTP, and dTTP. bA total of 35 cycles were performed for reactions with the exception of exon 8. cAnneling: 65°C 2 cycles; 64°C 10 cycles; 62°C 10 cycles, and 60°C 15 cycles. Table F in S1 File. Protocol for sequencing. aPerformed with 35 cycles. Table G in S1 File. Allele and genotype frequency of the ST3GAL1 gene polymorphisms in 68 patients infected with Influenza A(H1N1)pdm09. (DOCX)S1文件中的表A：针对rs113350588、rs1048479及其连锁不平衡（Linkage disequilibrium, LD）变异体的计算机模拟（in silico）功能分析结果。* 指代1000基因组计划中rs2142306、rs2736865与rs1048479多态性之间的连锁不平衡关系。S1文件中的表B：甲型H1N1流感（pdm09）感染患者体内ST3GAL1基因单倍型的频率分布。S1文件中的表C：甲型H1N1流感（pdm09）感染患者体内ST3GAL1基因双体型的频率分布。S1文件中的表D：用于ST3GAL1基因聚合酶链式反应（Polymerase Chain Reaction, PCR）扩增与核苷酸测序的引物序列。S1文件中的表E：PCR扩增实验方案。a 脱氧核糖核苷三磷酸混合物：dATP、dCTP、dGTP与dTTP。b 除第8外显子的扩增反应外，其余所有反应均设置35个循环。c 退火程序：65℃运行2个循环；64℃运行10个循环；62℃运行10个循环；60℃运行15个循环。S1文件中的表F：测序实验方案。a 反应共设置35个循环完成。S1文件中的表G：68例甲型H1N1流感（pdm09）感染患者中ST3GAL1基因多态性的等位基因与基因型频率。(DOCX)