CIL:20153

An ameba, Balamuthia mandrillaris, within a culture of monkey kidney feeder cells. Balamuthia extends from its branched pseudopodia while its rope-like body curves over and around a nucleus of a kidney cell; additional branches extended both downward and upward into and between other cells. Those branches terminate with more finger-like pseudopodia projections. The density and the movement of the cytoplasmic granules delineate the ameba from its feeder cells that, by comparison, are quiescent with few scattered cytoplasmic granules and a large, rounded nucleus containing several dense nucleoli. The purpose of this movie is to indicate the identity of an ameba amongst other cells in the co-culture. It shows the expanse of the ameba and its simultaneous involvement with several cells. Observations of the amebae under such conditions show their movement and interactions as they feed in a dimension unlike that of the ameba identification in sectioned material obtained from cases of amebic encephalitis. Details of the preparation can be found in CIL:19337.本影像展示了生长于猴肾饲养层细胞培养体系中的曼氏巴兰提亚阿米巴（Balamuthia mandrillaris）。该阿米巴伸出分枝状伪足（pseudopodia），绳索状的虫体弯曲覆盖并环绕肾细胞的细胞核；其额外分枝同时向上下两个方向延伸，侵入其他细胞并穿行于细胞间隙之间。这些分枝的末端形成更多指状伪足突起。胞质颗粒（cytoplasmic granules）的密度与运动状态可将该阿米巴与其饲养层细胞区分：饲养层细胞呈静止状态，仅含少量散在的胞质颗粒，且拥有一个大型圆形细胞核，内含数个致密核仁（nucleoli）。本影像的目的在于明确共培养体系中混杂于其他细胞间的阿米巴身份，展示了该阿米巴的延展范围，以及其同时与多个细胞发生相互作用的状态。在此类培养条件下对阿米巴进行观测，可记录其摄食过程中的运动与交互行为，这与从阿米巴性脑炎（amebic encephalitis）病例获取的切片材料中开展的阿米巴鉴定维度截然不同。该制片的详细信息可参见CIL:19337。