Additional file 1 of Construction of engineered yeast producing ammonia from glutamine and soybean residues (okara)

Additional file 1: Table S1. MRM conditions for the quantification of amino acids from soybean residues by LC–MS/MS. l-glutamine-13C5,15N2, and labeled amino acid standards [50 μg/mL algae hydrolysate amino acid mixture (U-13C, 97%–99%; U-15N, 97%–99%; Cambridge Isotope Laboratories CNLM-452-0.5)] as an IS were used for quantification of amino acids in pretreated soybean residues.

补充材料1：表S1。采用液相色谱-串联质谱（LC–MS/MS）定量分析大豆残渣中氨基酸的多反应监测（Multiple Reaction Monitoring，MRM）条件。以L-谷氨酰胺-13C5,15N2及同位素标记氨基酸标准品[50 μg/mL藻水解氨基酸混合物（U-13C，97%~99%；U-15N，97%~99%；剑桥同位素实验室CNLM-452-0.5）]作为内标（Internal Standard，IS），对预处理后的大豆残渣中的氨基酸进行定量分析。