Additional file 2: of Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells

A, Inhibitory effect of anti-ST8SiaII-IB and anti-ST8SiaIV-IB on metastasis of rhabdyomasarcoma cells after 4 weeks of tumor cell injection in mice. Mean values of each group of mice with corresponding standard deviation. Rhabdomyosarcoma cells expressing anti-ST8SiaII-IB and anti-ST8SiaIV-IB or anti-NCAM-IB or as negative control tumor cells stable transfected with the empty vector were injected intraperitoneally into 3 C57BL/6 J RAG-2 mice at a time. Luminiscence was determined at week 4 using in vivo imaging systems (IVIS). Shown are the results as luminiscence signals (p/sec/cm2/sr). ROI: region of interest. The red circles shows the metastic tumor cells in the region of lung and liver. B, Mean values of each group of mice with corresponding standard deviation. (XLS 1903 kb)

A、抗ST8SiaII-IB与抗ST8SiaIV-IB对小鼠瘤细胞接种4周后横纹肌肉瘤细胞转移的抑制效应。每组小鼠的均值及其对应标准差。将稳定转染抗ST8SiaII-IB、抗ST8SiaIV-IB、抗NCAM-IB，或空载载体（作为阴性对照）的横纹肌肉瘤细胞，一次性腹腔注射至3只C57BL/6 J RAG-2小鼠体内。于接种后第4周利用活体成像系统（in vivo imaging systems, IVIS）检测发光信号，结果以发光强度（p/sec/cm²/sr）呈现。ROI：感兴趣区域（region of interest）。红色圆圈标注了肺与肝区域内的转移性瘤细胞。B、每组小鼠的均值及其对应标准差。（附XLS文件，大小1903 kb）