CELLOIDS: pH-responsive DOPC Giant Unilamellar Vesicles with and without Linoleic Acid

As part of the CELLOIDS project, one of our main research focuses is exploring the feasibility of making the microrobot's membrane responsive to a pH gradient. Specifically, we investigated the incorporation of linoleic acid into Giant unilamellar vesicles composed of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine). The provided dataset compiles the diameters of liposomes prepared with DOPC (3.18 mM), DOPC:LA (linoleic acid) at a molar ratio of 100:1, and DOPC:LA at a molar ratio of 50:1. These liposomes were placed in buffers at both pH 6 and pH 7, simulating conditions similar to the tumor microenvironment. Our objective was to assess the stability of these liposomes under these conditions. In detail, we carefully prepared liposomes with varying molar ratios of DOPC and linoleic acid to determine the optimal amount of linoleic acid that induces membrane deformation while maintaining the structural integrity of the liposomes in acidic solutions. Subsequently, we subjected the liposomes to testing at the specified concentrations and compared their behavior under two different pH values. Finally, we observed the samples using a phase-contrast microscope immediately after the addition of the solutions and after a one-hour incubation period.作为CELLOIDS项目的一部分，本研究的核心方向之一是探索使微机器人（microrobot）膜响应pH梯度的可行性。具体而言，我们研究了将亚油酸（linoleic acid）掺入由DOPC（1,2-二油酰-sn-甘油-3-磷酸胆碱，1,2-dioleoyl-sn-glycero-3-phosphocholine）构成的巨型单层囊泡（Giant unilamellar vesicles）中的方案。本数据集收录了三种脂质体（liposomes）的粒径数据：分别为浓度3.18 mM的纯DOPC脂质体、DOPC与亚油酸摩尔比100:1的脂质体，以及DOPC与亚油酸摩尔比50:1的脂质体。我们将上述脂质体置于pH 6与pH 7的缓冲液中，以模拟类似肿瘤微环境（tumor microenvironment）的条件，旨在评估脂质体在该环境下的稳定性。详细而言，我们通过制备不同摩尔比的DOPC与亚油酸脂质体，以确定最优的亚油酸添加量——该添加量可在酸性溶液中诱导膜形变的同时，维持脂质体的结构完整性。随后，我们按指定浓度对脂质体开展测试，并对比其在两种pH条件下的行为表现。最终，我们分别在添加缓冲液后即刻，以及孵育1小时后，利用相差显微镜（phase-contrast microscope）对样本进行观察。