Revision of the cockroach subfamily Blattinae based on morphological and molecular analyses

Blattidae Latreille is widely distributed worldwide and includes a variety of sanitary pests, such as Periplaneta americana (Linnaeus), Periplaneta australasiae (Fabricius), and Periplaneta fuliginosa (Serville). The subfamily composition of Blattidae has undergone significant changes in recent years, and a few long-standing controversies have been resolved. However, the paraphyly of Blattinae Latreille, and the polyphyly of Periplaneta Burmeister within the subfamily, remain unresolved. We addressed these issues through phylogenetic analyses of the Blattinae based on mitochondrial genomes and nuclear genes (18S and 28S), and morphological analyses of nine male genital characters. Our study showed that Dorylaea Stål and Nazgultaure Lucanãs are sister taxa and distantly related to Blattinae. Periplaneta s.l. was found to be widely polyphyletic across five or six separate clades, while Mimosilpha Bey-Bienko was embedded within Homalosilpha Stål. Integrating ancestral character state recon..., , , # Revision of the cockroach subfamily Blattinae based on morphological and molecular analyses

[https://doi.org/10.5061/dryad.sn02v6xfm](https://doi.org/10.5061/dryad.sn02v6xfm)

## Description of the data and file structure

* \"Dataset 1\" comprised 17,176 nucleotide sites from 13 PCGs, four rRNA genes (12S, 16S, 18S, and 28S) and 22 tRNA genes.
* \"Dataset 2\" comprised 13 PCGs and four rRNA genes (12S, 16S, 18S, and 28S). 
* \"Dataset 1_IQ_partition\" and \"Dataset 2_IQ_partition\" are the best-fit substitution models obtained using PartitionFinder v.2.1.
* “IQ_TREE_Dataset 1.nex” and “IQ_TREE_Dataset 2.nex”  are the process files from IQ-TREE.
* “AUtest_IQ_partition.nex” is the process file from AU test.
* “ASR process file” is the process file from Phytools v.1.0-1.

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蜚蠊科（Blattidae Latreille）在全球范围内广泛分布，包含多种卫生害虫，例如美洲大蠊（Periplaneta americana (Linnaeus)）、澳洲大蠊（Periplaneta australasiae (Fabricius)）以及黑胸大蠊（Periplaneta fuliginosa (Serville)）。近年来，蜚蠊科的亚科组成发生了显著变化，多项长期存在的分类争议已得到解决，但蜚蠊亚科（Blattinae Latreille）的并系群问题，以及该亚科大蠊属（Periplaneta Burmeister）的多系群问题仍未得到厘清。本研究基于线粒体基因组与核基因（18S和28S）对蜚蠊亚科开展系统发育分析，并针对9个雄性生殖器性状进行形态学研究，以期解决上述分类难题。研究结果显示，Dorylaea Stål与Nazgultaure Lucanãs为姊妹群，且与蜚蠊亚科亲缘关系较远；广义大蠊属（Periplaneta s.l.）在5至6个独立演化支中呈现广泛的多系现象，而Mimosilpha Bey-Bienko嵌入于Homalosilpha Stål类群内部。本研究整合祖先性状状态重建……。本研究题为《基于形态与分子分析的蜚蠊亚科修订》（Revision of the cockroach subfamily Blattinae based on morphological and molecular analyses），相关数据集可访问链接：https://doi.org/10.5061/dryad.sn02v6xfm。

## 数据与文件结构说明
* 数据集1（Dataset 1）包含13个蛋白编码基因（PCGs）、4个核糖体RNA基因（12S、16S、18S和28S）以及22个转运RNA基因，共计17176个核苷酸位点。
* 数据集2（Dataset 2）包含13个蛋白编码基因与4个核糖体RNA基因（12S、16S、18S和28S）。
* Dataset 1_IQ_partition与Dataset 2_IQ_partition为使用PartitionFinder v.2.1筛选得到的最优替换模型。
* "IQ_TREE_Dataset 1.nex"与"IQ_TREE_Dataset 2.nex"为IQ-TREE的分析流程文件。
* "AUtest_IQ_partition.nex"为近似无偏检验（AU test）的分析流程文件。
* "ASR process file"为使用Phytools v.1.0-1完成的祖先性状状态重建流程文件。