In Situ Sequencing data used in "ISTDECO: In Situ Transcriptomics Decoding by Deconvolution"

This repository contains a few image slices from an in situ sequencing experiment [1]. The data is organized as follows: Each folder corresponds to a staining round. Each .tif image corresponds to a channel. The codebook can be loaded from the .mat file using MATLAB's load function or scipy.io.loadmat in Python. <strong>Acknowledgment:</strong> We kindly thank Qian et al. [1] for sharing their image data. <strong>References</strong> [1] Qian, Xiaoyan, et al. "Probabilistic cell typing enables fine mapping of closely related cell types in situ." Nature methods 17.1 (2020): 101-106.本仓库包含来自一项原位测序（in situ sequencing）实验的若干图像切片[1]。数据组织形式如下：每个文件夹对应一个染色轮次，每个.tif格式图像对应一个通道。可通过MATLAB的load函数或Python中的scipy.io.loadmat函数，从.mat格式文件中加载编码本（codebook）。 <strong>致谢：</strong>我们谨向Qian等人[1]分享其图像数据致以诚挚谢意。 <strong>参考文献</strong> [1] Qian, Xiaoyan 等. 概率细胞分型可实现密切相关细胞类型的原位精细定位[J]. 自然·方法（Nature Methods）, 2020, 17(1): 101-106.