Supplementary table, figures and DNA sequences of sorghum gene models SbiRTx430.01G455400 and SbiRTx.02G006600 that feature primers, gRNAs and indels created

In-context promoter bashing via genome editing is a route to identify and characterize critical regulatory regions that govern expression of genes of interest. The outcomes of in-context promoter bashing can be used to inform editing strategies to modulate the expression of selected gene models in a desired fashion. Here we employed in-context promoter bashing to characterize the proximal upstream regulatory regions of sorghum genes encoding phosphoenolpyruvate carboxykinase (Sb.PEPCK.BS, SbiTx430.01G455400) and alanine aminotransferase (SbiTx430.02G006600, SbAlaAT.BS), two proteins involved in the PCK C4 pathway. Characterized germinal edits within the targeted regions upstream of these two genes ranged in size from 138 bp up to 1790 bp. A 138 bp within the Sb.PEPCK.BS upstream region and a 1643 bp element within the Sb.AlaAT.BS upstream region were determined to be important for maintenance of transcription levels. No change in development or various physiological parameters was obser..., , , # Supplementary table, figures and DNA sequences of sorghum gene models SbiRTx430.01G455400 and SbiRTx.02G006600 that feature primers, gRNAs and indels created

[https://doi.org/10.5061/dryad.j3tx95xrv](https://doi.org/10.5061/dryad.j3tx95xrv)

## Description of the data and file structure

DNA sequence of edited sorghum gene models*Sb*.PEPCK.BS, SbiTx430.01G455400 & *Sb*AlaAT.BS SbiTx430.02G006600

Supplementary dataset document contains the supplemental Tables and Figures described in the manuscript.  

### Files and variables

#### File: Crop\_Sci\_DNA.zip

\**Description:***DNA sequences of sorghum gene models SbPEPCK.BS and Sb.AlaAT.BS with features highlighting edits created and various reagents used in the study (primers, gRNAs)*

## Code/software

none

## Access information

Data was derived from the following sources:

* The DNA sequece files are comprerssed/ SnapGene format (v 8.0.2). Supplementary dataset file is in MS word format (v.16.95).

,

依托基因组编辑的内源启动子敲除分析（in-context promoter bashing）是鉴定并表征调控目的基因表达的关键调控区域的有效手段。该分析的结果可用于指导编辑策略，以按需调控选定基因模型的表达水平。本研究采用该方法，对高粱中编码磷酸烯醇式丙酮酸羧激酶（phosphoenolpyruvate carboxykinase，Sb.PEPCK.BS，SbiTx430.01G455400）与丙氨酸氨基转移酶（alanine aminotransferase，SbiTx430.02G006600，SbAlaAT.BS）的基因的近端上游调控区域进行表征，这两种蛋白均参与PCK型C4光合途径。本研究在上述两个基因上游靶区域内鉴定得到的可遗传编辑片段大小介于138 bp至1790 bp之间。经分析确定，Sb.PEPCK.BS上游区域内的138 bp片段与Sb.AlaAT.BS上游区域内的1643 bp元件对维持基因转录水平至关重要。未观察到植株发育或各项生理参数出现显著变化。
# 包含引物、向导RNA（gRNA）及诱导插入缺失（indels）信息的高粱基因模型SbiRTx430.01G455400与SbiRTx.02G006600的补充表格、图表及DNA序列
https://doi.org/10.5061/dryad.j3tx95xrv

## 数据与文件结构说明
编辑后高粱基因模型Sb.PEPCK.BS（SbiTx430.01G455400）与Sb.AlaAT.BS（SbiTx430.02G006600）的DNA序列
补充数据集文档包含论文中提及的补充表格与图表。

### 文件与变量说明
#### 文件：Crop_Sci_DNA.zip
*描述：*包含高粱基因模型SbPEPCK.BS与Sb.AlaAT.BS的DNA序列，并标注了本研究中引入的编辑位点及所用各类试剂（引物、gRNA）的信息。

## 代码与软件
无

## 获取途径
本数据集源自以下来源：
* DNA序列文件为压缩格式，采用SnapGene（v8.0.2）格式；补充数据集文档采用Microsoft Word（v16.95）格式。