LMAS Figures

LMAS is an automated workflow enabling the benchmarking of traditional and metagenomic prokaryotic de novo assembly software using defined mock communities. As proof of concept, the eight bacterial genomes and four plasmids, in tripled form, of the ZymoBIOMICS Microbial Community Standards are made available to be used as reference. Simulated raw sequence data of the mock communities with evenly distributed reads, generated from the reference genomes, with and without error, were obtained with inSilicoSeq. Project repository: https://github.com/cimendes/LMASLMAS是一款自动化工作流，可用于对传统组装软件与宏基因组原核生物从头组装（de novo assembly）软件开展基准测试，测试所用的数据集为预设的模拟微生物群落。作为概念验证，本研究提供了ZymoBIOMICS微生物群落标准品中的8个细菌基因组与4个质粒的三份重复样本作为参考数据集。研究人员借助inSilicoSeq工具，基于上述参考基因组生成了两种模拟原始测序数据：一种是读长分布均匀的无错误模拟测序数据，另一种是带有测序错误的模拟测序数据。本项目的代码仓库地址为：https://github.com/cimendes/LMAS