Global 5mC and 5hmC levels in sixteen pairs of HCC and non-HCC samples and two HCC cell lines investigated by UPLC-MS/MS

We performed UPLC-MS/MS to investigate global 5mC and 5hmC levels in sixteen pairs of HCC and non-HCC samples and two HCC cell lines (97L and LM6 cells). We found that 5mC and 5hmC were frequently decreased in 71% and 100% of the HCC specimens, respectively, compared with non-HCC specimens. Correspondingly, 5mC and 5hmC levels in the two HCC cell lines remained relatively low.

我们采用超高效液相色谱-串联质谱（UPLC-MS/MS），对16对肝细胞癌（HCC）与非肝细胞癌（non-HCC）样本，以及2株肝细胞癌细胞系（97L细胞与LM6细胞）中的全基因组5-甲基胞嘧啶（5mC）和5-羟甲基胞嘧啶（5hmC）水平开展了检测分析。结果显示，相较于非肝细胞癌样本，分别有71%和100%的肝细胞癌标本中5mC与5hmC水平显著降低；与之对应，这两株肝细胞癌细胞系内的5mC和5hmC水平同样始终维持在较低水平。