Additional file 1 of Comprehensive analysis and expression characterization of potato Cytochrome P450 gene family and the role of StCYP67 in abiotic stresses
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Additional file 1. Figure S1. Phylogenetic relationship, conserved motifs and gene structure of StCYP450 genes. A Phylogenetic relationship of StCYP450 genes. B The conserved motifs of StCYP450 genes were showed in different colors. C The exon-intron structure. The coding sequence (CDS) and untranslated region (UTR) were showed in different colors, and the lines between the boxes mean introns. Figure S2. The chromosomal mapping analysis of StCYP450 gene family in potato. Table S1. Physical and chemical properties of StCYP450 gene family in potato. AA. amino acid sequence length; MW. molecular weight; pI. isoelectric point; GRAVY. grand average of hydropathicity; II. instability index; AI. aliphatic index; SL. subcellular localization; CP. chloroplast; C. cytoplasm; CY. cytoskeleton; N. nucleus; V. vacuole; M. mitochondrion; PM. plasma membrane; E. extracellular matrix; ER. endoplasmic reticulum; P. peroxisome; G. golgi apparatus; C_N. cytoplasm_nucleus. Table S2. String protein in Arabidopsis corresponding to CYPs gene family in potato of phylogenetic tree. Table S3. Functional annotation of StCYP450 related to BRs and ABA in potatoes. Table S4. The GO classification of the annotated StCYP85 clan genes in potato. Table S5. FPKM values of StCYP72 and StCYP85 genes in various potato tissues. Table S6. FPKM values of StCYP72 and StCYP85 genes in potato different treatments. Table S7. Sequences of primer employed in the amplification of StCYP67 gene and qRT-PCR analysis.
附加文件1。图S1。马铃薯StCYP450基因的系统发育关系、保守基序与基因结构。A:StCYP450基因的系统发育关系;B:以不同颜色展示StCYP450基因的保守基序;C:外显子-内含子结构。编码序列(CDS)与非翻译区(UTR)以不同颜色标注,方框间的连线代表内含子。图S2。马铃薯StCYP450基因家族的染色体定位分析。表S1。马铃薯StCYP450基因家族的理化性质。其中缩写含义如下:AA:氨基酸序列长度;MW:分子量;pI:等电点;GRAVY:总亲水性平均系数;II:不稳定指数;AI:脂肪族指数;SL:亚细胞定位;CP:叶绿体;C:细胞质;CY:细胞骨架;N:细胞核;V:液泡;M:线粒体;PM:质膜;E:细胞外基质;ER:内质网;P:过氧化物酶体;G:高尔基体;C_N:细胞质-细胞核。表S2。系统发育树中与马铃薯CYP450基因家族对应的拟南芥STRING蛋白。表S3。马铃薯中与油菜素甾醇(BRs)和脱落酸(ABA)相关的StCYP450基因功能注释。表S4。马铃薯中已注释的StCYP85家族基因的基因本体(GO)分类。表S5。马铃薯不同组织中StCYP72与StCYP85基因的FPKM值。表S6。不同处理条件下马铃薯StCYP72与StCYP85基因的FPKM值。表S7。用于StCYP67基因扩增及实时荧光定量PCR(qRT-PCR)分析的引物序列。



