Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain BL21(DE3)/pCC90 D54-STOP-LB, LB+0.2% glucose, LB+0.5% glucose

Microbial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition of the growth medium affects the movement and formation of aggregates of recombinant Escherichia coli strains.
This study focused on several recombinant strains: BL21(DE3)/pCC90, BL21(DE3)/pACYCpBAD, BL21(DE3)/pCC90 Dra D-mut, BL21(DE3)/pCC90 D54-STOP, AAEC191A/pCC90, AAEC191A /pACYCpBAD, AAEC191A/pCC90 Dra D-mut and AAEC191A/pCC90 D54-STOP. The experimental setup involved transferring a small portion of overnight cultures of these recombinant strains to various media compositions. These media include PBS, PBS + 0.2% glucose, PBS + 0.5% glucose, LB, LB + 0.2% glucose, LB + 0.5% glucose, M9 + 0.5% cassaminic acids, M9 + 0.5% cassaminic acids + 0.2% glucose and M9 + 0.5% cassaminic acids + 0.5% glucose.
The catalog contains original video data that can be played using Olympus' CellSense software. The videos contain full layers of data characterizing the image recording process.

微生物运动性是诸多微生物生命周期的核心特征，亦是帮助微生物在多样且动态的环境中定向穿梭的关键生存机制。该现象在应对时空尺度上的刺激变化时显得尤为重要。本实验旨在探究培养基组分如何影响重组大肠杆菌（Escherichia coli）菌株的运动性与聚集体形成。
本研究聚焦于以下数株重组菌株：BL21(DE3)/pCC90、BL21(DE3)/pACYCpBAD、BL21(DE3)/pCC90 Dra D-mut、BL21(DE3)/pCC90 D54-STOP、AAEC191A/pCC90、AAEC191A /pACYCpBAD、AAEC191A/pCC90 Dra D-mut及AAEC191A/pCC90 D54-STOP。
实验设置为：将上述重组菌株的过夜培养物少量接种至不同组分的培养基中。所用培养基包括磷酸盐缓冲液（PBS）、PBS + 0.2%葡萄糖、PBS + 0.5%葡萄糖、LB培养基（LB）、LB + 0.2%葡萄糖、LB + 0.5%葡萄糖、M9培养基 + 0.5%酪蛋白氨基酸（cassaminic acids）、M9培养基 + 0.5%酪蛋白氨基酸 + 0.2%葡萄糖以及M9培养基 + 0.5%酪蛋白氨基酸 + 0.5%葡萄糖。
本数据集包含原始视频数据，可通过奥林巴斯（Olympus）的CellSense软件播放。所有视频均涵盖表征图像录制全流程的全层级数据。