Data sets for Perspective: SARS-CoV-2's potential mechanism of regulating cellular responses through depletion of specific host miRNAs

Using a bioinformatic analysis of human miRNA potential interactions with the SARS-CoV-2’s genome, we examined the potential miRNA target sites in 7 coronavirus genomes that include SARS-CoV-2, MERS-CoV, SARS-CoV, and 4 non-pathogenic coronaviruses. (<strong>Data Set 1</strong>). <em>Our approach was to examine and compare 3 pathogenic and 4 non-pathogenic strains of HCoVs. The HCoVs' RNA genomes of pathogenic strains were SARS-CoV-2 (NC_045512.2), SARS-CoV (NC_004718.3), MERS-CoV (NC_019843.3). The non-pathogenic strains were HCoV-OC43 (KU131570.1), HCoV-229E (NC_002645.1), HCoV-HKU1 (KF686346.1), and HCoV-NL63 (NC_005831.2). These coronaviruses were tested against the set of 896 confident mature human miRNA sequences that were obtained from the miRBbase v2.21 using the RNA22 v2 microRNA target discovery tool web-server. In order to reduce the false discovery rate of the MTS predictions, the most strict parameters were applied to the default computation workflow using a specificity of 92% versus a sensitivity of 22%.</em> <em>In <strong>Data set 2</strong>, using the miRDIP database with only top 1% of the most probable targets considered, we analyzed the potential targets of miRNA that could be bound to either the pathogenic, the non-pathogenic or both groups of HCoVs. </em> In <strong>Data set 3</strong>, using miRNAFold webserver we identified 10 pre-miRNA sequences in the <em>SARS-CoV-2</em> RNA sequence that could potentially enter the human RNAi pathway. The graphical summary of our working hypothesis is provided in the<strong> graphical abstract</strong>.通过对人类微小RNA（miRNA，microRNA）与严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）基因组的潜在相互作用开展生物信息学分析，我们对包含SARS-CoV-2、中东呼吸综合征冠状病毒（MERS-CoV）、严重急性呼吸综合征冠状病毒（SARS-CoV）以及4种非致病性冠状病毒在内的7种冠状病毒基因组中的潜在miRNA靶位点进行了检测，即**数据集1**。*本研究的研究思路为检测并比较3株致病性人类冠状病毒（Human Coronavirus, HCoV）与4株非致病性人类冠状病毒毒株：致病性毒株的RNA基因组分别为SARS-CoV-2（登录号NC_045512.2）、SARS-CoV（登录号NC_004718.3）以及MERS-CoV（登录号NC_019843.3）；非致病性毒株则为HCoV-OC43（登录号KU131570.1）、HCoV-229E（登录号NC_002645.1）、HCoV-HKU1（登录号KF686346.1）与HCoV-NL63（登录号NC_005831.2）。本研究针对上述冠状病毒，借助RNA22 v2微小RNA靶标发现工具网页服务器，对从miRBbase v2.21数据库获取的896条高质量成熟人类miRNA序列集合开展了靶标验证检测。为降低微小RNA靶位点（microRNA target site, MTS）预测的假发现率，本研究在默认计算流程中采用了最为严格的参数，将特异性设为92%，灵敏度设为22%。*在**数据集2**中，*我们仅选取miRDIP数据库中排名前1%的最具可能性的靶标，分析了可结合致病性人类冠状病毒、非致病性人类冠状病毒或同时结合这两类冠状病毒的miRNA潜在靶位点。*在**数据集3**中，我们通过miRNAFold网页服务器在*SARS-CoV-2*的RNA序列中鉴定出10条可能参与人类RNA干扰（RNA interference, RNAi）通路的前体miRNA序列。本研究的工作假说图形化总结已在**图形摘要**中呈现。