CIL:12580

Confocal laser scanning micrograph of immunofluorescence-labeled corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green) in whole mount preparations of its leafhopper vector D. maidis, showing the anterior and posterior midgut. Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CIL:19118, from the same image group, is a labeled version of this. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.本共聚焦激光扫描显微图像展示了媒介昆虫玉米叶蝉（*Dalbulus maidis*，简称D. maidis）整装制片中经免疫荧光标记的玉米矮化螺原体（Corn stunt spiroplasma, CSS，*Spiroplasma kunkelii*，柔膜菌纲（Mollicutes））聚集体（呈绿色），涵盖该昆虫的前中肠与后中肠。该整装器官先以CSS特异性一抗进行标记，随后结合荧光二抗（Alexa Fluor-488，呈绿色），再使用核染料碘化丙啶（propidium iodide，呈红色）完成复染。同图像组中的CIL:19118为此图像的标注版本。CSS为一类无细胞壁原核生物，可引发玉米矮化病，可通过部分叶蝉物种实现生物传播，例如*Dalbulus maidis*与*D. gelbus*（半翅目Hemiptera，叶蝉科Cicadellidae）。螺原体细胞仅由单位膜包裹，无细胞壁，可呈近球形、多形性或维持丝状/螺旋状形态。CSS可在植物与昆虫宿主体内增殖，并在媒介昆虫取食新宿主植物时随唾液分泌物完成传播。更多详细信息可参考Ammar与Hougenhout于2005年发表于《Annals of the Entomological Society of America》第98卷第6期的论文，页码为820–826。