Quantitative phytoplankton profiles for Pensacola Transect Station P9 collected during the R/V Bellows cruise of DeSoto Canyon, April 6, 2013

This dataset reports phytoplankton species abundance data for samples collected on 2013-04-06 onboard the R/V Bellows BE_1317 cruise in the Gulf of Mexico from 2013-04-06 to 2013-04-08. Samples were taken at Pensacola Transect Station P9 (29.41667 N, 87.25 W) in the Florida Panhandle Bight at the head of DeSoto Canyon. One-liter samples were collected from the surface and at depths of 50, 100, 150, 200, and 475 meters using Niskin bottles. Up to one liter of each sample was filtered onto a 0.45-micrometer nitrocellulose filter and air-dried. A portion of each filter was mounted on an aluminum stub, sputter-coated, and examined with a JEOL JSM-6480 LV scanning electron microscope. Diatoms, coccolithophores, and armored dinoflagellates were identified to the lowest possible taxonomic category. The number of cells of each taxon in a field of view at a magnification of 1000x was recorded, with up to 700 fields-of-view examined, depending on the density of phytoplankton in the sample. The dataset includes the depth, the volume filtered, the number of cells of each taxon counted, and the calculated abundance of each taxon in cells per liter. Micrographs taken during the analysis are included.

本数据集收录了2013年4月6日至8日期间，在墨西哥湾开展的R/V Bellows号BE_1317航次中，于2013年4月6日采集的水样的浮游植物物种丰度数据。采样点位位于德索托峡谷头部的佛罗里达潘汉德尔湾彭萨科拉断面P9站（29.41667°N，87.25°W）。研究人员采用尼斯金采水器（Niskin bottles），分别采集该点位表层及50、100、150、200、475米水深的1升水样。每份水样取至多1升体积，经0.45微米硝酸纤维素滤膜（nitrocellulose filter）过滤后风干。将每张滤膜的一部分粘贴至铝样品台（aluminum stub）上，经喷金镀膜处理后，使用JEOL JSM-6480 LV型扫描电子显微镜（scanning electron microscope）进行观测。将硅藻（diatoms）、颗石藻（coccolithophores）及被甲甲藻（armored dinoflagellates）鉴定至尽可能低的分类阶元。记录1000倍放大视野下各分类单元的细胞数，依据水样中浮游植物的密度，最多可观测700个视野。本数据集包含采样水深、过滤水样体积、各分类单元的计数细胞数，以及以每升细胞数为单位计算的各分类单元丰度，同时附带分析过程中拍摄的显微照片。