ZIP-file of fluorescent 3D image stacks of matrix-embedded prostate cancer cell co-cultures (WPMY-1) treated with docetaxel (0.003 uM)

ZIP-file containing confocal image stacks from LNCaP (RFP) 3D co-cultures (WPMY-1, GFP) embedded in Matrigel-collagen and grown in 96-well plates. The cultures are stained with Hoechst and EdU and are treated with docetaxel (0.003 uM) and imaged at day 20. Images are 16-bit multi-channel image stacks containing the EdU, RFP, GFP and Hoechst channel (in that order) . The pixelsize is ( 1.3 x 1.3 x 10 ) micron at 10x magnification. Images are in TIF-format, and are best opened in ImageJ for visualisation.

本数据集为ZIP压缩包，内含源自LNCaP（红色荧光蛋白（RFP）标记）与WPMY-1（绿色荧光蛋白（GFP）标记）的3D共培养体系的共聚焦图像栈。该共培养体系包埋于基质胶（Matrigel）-胶原基质中，于96孔板内培养；培养体系经赫斯特荧光染料（Hoechst）与5-乙炔基-2'-脱氧尿苷（EdU）染色，并经0.003 μM多西他赛（docetaxel）处理，于培养第20天完成成像。图像为16位多通道图像栈，按顺序包含EdU、RFP、GFP及Hoechst四个通道。在10倍放大倍率下，像素尺寸为(1.3 × 1.3 × 10) 微米。所有图像采用TIF格式存储，推荐使用ImageJ软件进行可视化查看。