DATASET of Spatial Transcriptomics Mouse Hippocampal Slices for MEA-seqX Platform

Sequencing data (fastq.gz) was processed using the 10X Genomics Space Ranger Count pipeline. L75027 = Mouse_Hippocampus_SD L75031 = Mouse_Hippocampus_ENR Outputs include a spatial folder containing outputs that capture the spatiality of the data and a filtered_feature_bc matrix folder containing the tissue-associated barcodes in MEX format. spatial/ aligned_fiducials.jpg: Aligned fiducials QC image detected_tissue_image.jpg: Detected tissue QC image scalefactors_json.json: Scale conversion factors for spot diameter and coordinates at various image resolutions tissue_hires_image.png: Downsampled full resolution image tissue_lowres_image.png: Full resolution image downsampled to 600 pixels on the longest dimension tissue_positions_list.csv: CSV containing spot barcode filtered_feature_bc_matrix.h5: Contains only tissue-associated barcodes in in HDF5 format filtered_feature_bc_matrix/ barcodes.tsv.gz: List of barcodes features.tsv.gz: List of features matrix.mtx.gz: Count matrix测序数据（fastq.gz格式）通过10X Genomics的Space Ranger计数流程完成处理。 L75027对应小鼠海马体SD组（Mouse_Hippocampus_SD），L75031对应小鼠海马体ENR组（Mouse_Hippocampus_ENR）。 输出结果包含spatial文件夹与filtered_feature_bc matrix文件夹两类目录：spatial文件夹存储用于捕获数据空间特征的相关输出，filtered_feature_bc matrix文件夹内含MEX格式的组织关联条形码。 spatial/ aligned_fiducials.jpg：对齐基准标记质控图像 detected_tissue_image.jpg：组织检测质控图像 scalefactors_json.json：不同图像分辨率下的斑点直径与坐标缩放转换因子 tissue_hires_image.png：降采样全分辨率图像 tissue_lowres_image.png：最长边降采样至600像素的全分辨率图像 tissue_positions_list.csv：包含斑点条形码信息的CSV文件 filtered_feature_bc_matrix.h5：仅包含HDF5格式的组织关联条形码 filtered_feature_bc_matrix/ barcodes.tsv.gz：条形码列表 features.tsv.gz：特征列表 matrix.mtx.gz：计数矩阵