<b>Call characteristics of five </b><b><i>Kurixalus</i></b><b> </b><b>species (Anura: Rhacophoridae)</b>

Species-specific acoustic signals are considered a prezygotic isolation mechanism, and comparative bioacoustical analyses of animal vocalizations facilitate species identification. In the present study, we described and compared the acoustic characteristics of <i>Kurixalus</i> <i>hainanus</i>,<i> K. odontotarsus</i>,<i> K. lenquanensis</i>,<i> K. qionglaiensis</i> and <i>K. idiootocus</i>.<i> </i>A characteristic spectrogram and oscillogram are presented for each species, together with information about the spectral and temporal characteristics of the calls. We found that the calls of each species comprised three note types, and there were significant differences in the acoustic characteristics of the three note types. There were also significant interspecific differences in the dominant frequency, note duration, call duration, mean number of notes, and proportion of notes for each note type. In addition, the five species differed in body mass, snout-vent length and head width. The factors influencing the differences in vocalizations among the five <i>Kurixalus</i> species require further study. We provided the dominant frequency (DF), note duration (ND), inter-note intervals (IV), call duration (CD) and mean number of notes (MN) of three call types of these species, and discussed the interspecific variation in calls, aiming to inform future ecological studies of the species and taxonomic studies in the genus <i>Kurixalus</i>.物种特异性声学信号被视为合子前隔离机制，针对动物发声开展比较生物声学分析可助力物种鉴定。本研究描述并比较了海南溪树蛙（Kurixalus hainanus）、齿腺溪树蛙（K. odontotarsus）、泉林溪树蛙（K. lenquanensis）、邛崃溪树蛙（K. qionglaiensis）以及宽足溪树蛙（K. idiootocus）的声学特征。本研究为各物种提供了典型声谱图（spectrogram）与波形图（oscillogram），并附带其鸣唱的频谱及时域特征信息。研究发现，各物种的鸣唱均包含三种音节类型，且三类音节的声学特征均存在显著差异。此外，各类音节的主频（dominant frequency）、音节时长（note duration）、鸣唱时长（call duration）、平均音节数（mean number of notes）以及音节占比均存在显著的种间差异。除此之外，上述五个物种在体重、吻肛长以及头宽方面亦存在差异。影响这五种溪树蛙属物种鸣唱差异的具体因素仍有待进一步探究。本研究提供了上述物种三类鸣唱类型的主频（dominant frequency, DF）、音节时长（note duration, ND）、音节间隔（inter-note intervals, IV）、鸣唱时长（call duration, CD）以及平均音节数（mean number of notes, MN），并探讨了鸣唱的种间变异，旨在为该类群后续的物种生态学研究以及溪树蛙属的分类学研究提供参考。