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Human buffy coat gene expression, custom 250-plex Nanostring panel

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE124549
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Buffy coat PBMC RNA expression was tested using a custom Nanostring probe panel, to identify general immune and glucocorticoid receptor-associated immune genes. ClinicalTrials.gov Identifier: NCT00824941 Buffy coat samples were collected using Lymphoprep density gradient protocol, total RNA extracted with miRVana kit. Custom 250-plex "ImmunoGC" panel selected genes for: 1) reported glucocorticoid immune responsiveness, 2) immune-cell type specific expression, 3) microRNA processing, and 4) putative stress-associated biomarkers.
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2020-01-31
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