Reconstruction of large genomic regions from single organisms and metagenomic samples

To overcome limitations related to genome reconstruction and to facilitate genomic diversity exploration of model and non-model organisms, we developed a target enrichment method for large genomic regions that could be applied to single organisms or metagenomic samples. Based on a hybridization capture strategy, this method allows for the enrichment of tens of kbp flanking a short, targeted DNA sequence. Due to the large DNA fragments captured, this innovative approach allows the resolution of complex genetic organization with the presence of repeats or genetic variations that are highly difficult to characterize using current molecular approaches. When applied to metagenomic samples, this approach enables the assignment of identity and function to micro-organisms in natural environments, one of the ultimate goals of microbial ecology.