Additional file 4 of Detection and discovery of plant viruses in soybean by metagenomic sequencing

Additional file 4 Figure S4. Plant viruses confirmed by Sanger sequencing in field grown soybean plants. Reverse transcription-polymerase chain reaction (RT-PCR) was performed in 78 field samples. The PCR products were visualized in a 1% agarose gel electrophoresis containing SYBR Safe DNA gel stain. The PCR products were cleaned using ExoSAP-IT PCR product cleanup reagent and sequenced on Sanger sequencing platform. Primer sequences were designed using Primer3 plus from assembled contigs and the primers were used in this study.附加文件4 图S4：田间种植大豆植株中经桑格测序（Sanger sequencing）验证的植物病毒。本研究对78份田间样本开展了逆转录聚合酶链式反应（Reverse transcription-polymerase chain reaction, RT-PCR）；PCR产物在含有SYBR Safe DNA凝胶染料的1%琼脂糖凝胶电泳中进行可视化检测；随后使用ExoSAP-IT PCR产物纯化试剂对PCR产物进行纯化，并通过桑格测序平台完成测序；引物序列通过Primer3 plus软件基于组装得到的重叠群（contigs）设计，并应用于本研究。