Comparison of MyoD binding sites in WT and Arp5-KO RD cells [ChIP-seq]

Actin-related protein 5 (Arp5) is a nuclear localized actin-like protein. Arp5 is relatively underexpressed in muscle tissues such as heart and aorta, where it plays a role in inhibiting muscle differentiation by interacting with myocardin. In skeletal muscle, Arp5 was also found to be underexpressed and play an inhibitory role by competitively inhibiting the interaction between MyoD and Pbx/Meis. ChIP-seq analysis demonstrate that MyoD was actively accumulated at its target loci in Arp5 knockout rhabdomyosarcoma RD cells compared to parental RD cells; and furthermore, the consensus binding motifs of Pbx/Meis were enriched in the ChIP peaks.