Additional file 2 of Sequential host-bacteria and bacteria-bacteria interactions determine the microbiome establishment of Nematostella vectensis

Additional file 2: Table S1. Bacterial strains isolated from Nematostella vectensis with phylogeny according to GenBank Accession number, and ASV names. Table S2. Clusters with the genomes (self-sequenced or downloaded from the ncbi database) which were used for the metabolic potential analysis. Table S3. Count table for the ASVs for the recolonization of polyps and tubes. Table S4. Taxonomy of the ASVs. Table S5. Pairwise Permanova on the Bray–Curtis distances for the recolonization of polyps. Table S6. Pairwise Permanova on the Bray–Curtis distances for the recolonization of tubes. Table S7. ASVs with shortened ASV number and 97% cluster to which they belong. Table S8. Bacterial strains from which genomes were sequenced, with developmental stage from which they were isolated, phylogeny and ncbi classification. Table S9. Pathways contributing the most to the separation on dimension 1 in the PCA showing the metabolic capabilities during recolonization (Fig. 4A). Table S10. Up- and downregulated genes upon recolonization with bacteria, independent of the inoculum (bL-bJ-bA vs GF). Table S11. Top20 upregulated genes upon recolonization with bacteria, independent of the inoculum. All relevant scripts can be found on GitHub under https://github.com/Porthmeus/DominEtAl_NematostellaRecolonization and https://github.com/ikmb/assembly-bacteria

附加文件2：表S1：分离自艾氏海葵（*Nematostella vectensis*）的细菌菌株，其系统发育信息依据GenBank登录号，以及扩增子序列变异（Amplicon Sequence Variant, ASV）名称。表S2：用于代谢潜能分析的基因组簇（包含自主测序或从国家生物技术信息中心（National Center for Biotechnology Information, NCBI）数据库下载的基因组）。表S3：用于水螅体与螅管再定殖的扩增子序列变异计数表。表S4：扩增子序列变异的分类学注释信息。表S5：针对水螅体再定殖的Bray–Curtis距离进行的两两置换多元方差分析（Permutational Multivariate Analysis of Variance, PERMANOVA）结果。表S6：针对螅管再定殖的Bray–Curtis距离进行的两两置换多元方差分析结果。表S7：带有简化编号的扩增子序列变异及其归属的97%相似性聚类簇。表S8：完成基因组测序的细菌菌株信息，包含其分离来源的发育阶段、系统发育信息以及NCBI分类学注释。表S9：在主成分分析（Principal Component Analysis, PCA）第一维度上对组间分离贡献最大的代谢通路，该分析展示了再定殖过程中的代谢能力（对应图4A）。表S10：无论接种物来源如何，经细菌再定殖后上调与下调的基因列表（bL-bJ-bA vs 无菌组（GF））。表S11：无论接种物来源如何，经细菌再定殖后上调的前20位基因列表。所有相关脚本可在GitHub平台获取，链接为https://github.com/Porthmeus/DominEtAl_NematostellaRecolonization 与 https://github.com/ikmb/assembly-bacteria