ELISA data for the 50 most reactive M. catarrhalis peptides – Average ELISA titers for groups of sera.

The peptides are named by the ORF followed by a number indicating the individual peptide for the respective ORF. Individual sera were obtained from asthma patients and healthy individuals and convalescent sera patients with otitis media (OM). Listed are the 50 peptides with highest average ELISA units of the 402 peptides analyzed. ELISA units were calculated as 1,000×[(A405 wells with serum) – (A405 wells with secondary antibody alone)]. The serum ELISA units were additionally corrected for the background reactivity of sera with streptavidin, by subtracting the values obtained with streptavidin coated wells in the absence of peptide from the values obtained in the wells containing bound peptides.本数据集所包含的肽段以开放阅读框（Open Reading Frame，ORF）命名，其后跟随的数字用于标识对应开放阅读框的单条肽段。实验血清分别采集自哮喘患者、健康个体，以及中耳炎（Otitis Media，OM）康复患者的恢复期血清。本次分析共涵盖402条肽段，以下列出的是其中平均酶联免疫吸附测定（Enzyme-Linked Immunosorbent Assay，ELISA）单位最高的50条肽段。ELISA单位的计算公式为：1000×[(添加血清的检测孔A405吸光度值) – 仅添加二抗的检测孔A405吸光度值]。为消除血清与链霉亲和素的非特异性结合背景干扰，我们以包被肽段的检测孔所得吸光度值，减去无肽段包被的链霉亲和素包被孔的吸光度值，对血清ELISA单位进行了额外校正。