CIL:912

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Stereo pair with CIL:911.本数据集的实验样本为兔腰大肌骨骼肌纤维（Rabbit psoas skeletal muscle fibers），先将其固定于恒定长度，并通过浸入甘油诱导肌纤维僵直。随后将纤维置于Heuser型冷冻压片机（Heuser-type cryopress）中，通过与液氦冷却铜块接触实现快速冷冻；接着在Balzers 400型冷冻断裂仪（Balzers 400 freeze fracture machine）中完成冷冻蚀刻。之后在24°条件下采用铂-碳（Pt-C）对样本进行旋转复型，再以加速电压100 keV的JEOL 100CX2型透射电子显微镜（JEOL 100CX2 transmission electron microscope）进行成像观察。以75000倍放大倍率将图像记录于柯达（Kodak）4489胶片上，并以±5°倾角拍摄立体图像对。最后使用尼康（Nikon）Coolscan 9000ED扫描仪以20微米的像素间距扫描胶片，该数据集对应的立体图像对编号为CIL:911。