Data from: Correlation of shell phenotype and local environment suggests a role for natural selection in the evolution of Placostylus snails

Set Up Spread Sheet-Codes Barcodes etcSpreadsheet describing individuals species and collection location. Second sheet describes the inline barcodes and illumina index used for demultiplexing. Third sheet has Long Lat information for samplesSetUpSpreadSheet.xlsxMorphometric DataMorphometric Dataset. Coordinates and Species/Population/Code classifiersPlacoAllinSlidV2.txtND2 Sequences Placostylus Aligned100 individuals ND2 sequence. Codes match spreadsheetND2Placo.phyND2 assembled GeneAssembly of placostylus mtDNA reads. Covers ND2, CO1, ND4, ND3,COX3ND2assembledGene.fastaMain SNP file STRUCTURE formatDataset of 3764 SNP structure file. Population information included in second column.MainDataSetSTRUCTUREfile.txtNon Neutral SNP file STRUCTURE formatResults from Bayescan non-neutral SNP loci detected. Population ID included in STRUCTURE fileNonNeutralSNPstructure.txtEnvironmental DataEnvironmental Data for populations used on correlation analysisEnvirodatagrandTerreOvAlldatapops.csvLargest SNP dataset statistics results from Stacksparameters set -m 8 –N 2 –M 4 –n 3 -t. A filtered version of this dataset was used in analysis in paper. This dataset required SNPs occur across 2 populations at 90% to be called.Archive 2.zipSTACKS dataset statistics dataset Number 2parameters set -m 8 –N 2 –M 4 –n 3 -t. This dataset required SNP occur across all populations at any coverageArchive.zipStructure file for sympatric populationsSTRUCTURE file for sympatric populationsstructureSYMPATRICpopNOSINGLESp2r5sym.txtSTACKS dataset statistics for sympatric datasetStacks raw statistics for sympatric datasetArchive.zipSTACKS dataset statistics dataset Number 3Raw statistics for SNP dataset number 3 (settings -m 8 –N 2 –M 4 –n 3 -t). This dataset required SNPs to occur in 90% of individuals across 3 populations before being calledArchive.zipIndex_E part 1 Raw RAD readsRaw RAD reads. Barcodes and Codes can be found in SpreadsheetArchive.zipIndex e part 2 Raw RAD readsSee spread sheet for individual details. De multiplexed Index E part 2.Archive 2.zipIndex_F part 1 Raw RAD readsIndex_F part 1 raw RAD reads. Codes correlate to spreadsheet.Archive.zipIndex_F part 2 Raw RAD readsIndex_F part 2 Raw RAD reads. Codes and barcodes located in spreadsheetArchive 2.zipIndex_G part 1 Raw RAD readsIndex_G part 1 Raw RAD reads. Codes in spreadsheet.Archive.zipIndex_G part 2 Raw RAD readsIndex_G part 2 Raw RAD reads. Codes in spreadsheetArchive 2.zipIndex_H part 1 Raw RAD readsIndex_H part 1 Raw RAD reads. Codes in SpreadsheetArchive.zipIndex_H part 2 Raw RAD readsIndex_H part 2 Raw RAD reads. Codes in SpreadsheetArchive 2.zipIndex_I part 1 Raw RAD readsIndex_I part 1 Raw RAD reads. Codes in spreadsheetArchive.zipIndex_I part 2 Raw RAD readsIndex_I part 2 Raw RAD reads. Codes in spreadsheetArchive 2.zipForward Read Genomic DNAForward Read used to assembled partial mtDNA genome of Placostylus. Sample from Mépouiri. Sequenced HiSeq120223_I238_FCC0HH4ACXX_L5_SZAXPI005962-142_1.fq.gzReverse Read Genomic DNAReverse Read. Genomic DNA placostylus120223_I238_FCC0HH4ACXX_L5_SZAXPI005962-142_2.trimmed.paired.fq.gzRaxMLTreeBSTRAPplacoND2Raw tree file from RaxML of ND2 genePlacostylus Morphometric Data Better explainedSee comments in file for explanation. Includes the same data as the other morphological data file but is better explained. Includes Raw and Procrustes Distance co-ordinates.PlacostylusMorphometricData.xlsx

1. 设置电子表格-条码（Barcodes）等：该电子表格记录了个体、物种及采样地点信息，第二个工作表记录了用于测序解复用（demultiplexing）的内置条码与Illumina索引（Illumina index）信息，第三个工作表包含样本的经纬度坐标。对应文件：SetUpSpreadSheet.xlsx
2. 形态测量数据（Morphometric Data）：形态测量数据集，包含坐标及物种/种群/编码分类器。对应文件：PlacoAllinSlidV2.txt
3. ND2序列（Placostylus属比对序列）：包含100个个体的ND2序列，编码信息与电子表格一致。对应文件：ND2Placo.phy
4. ND2组装基因：布纹蜗牛属（Placostylus）线粒体DNA（mitochondrial DNA, mtDNA）读段的组装结果，覆盖ND2、CO1、ND4、ND3、COX3基因。对应文件：ND2assembledGene.fasta
5. 主单核苷酸多态性（Single Nucleotide Polymorphism, SNP）文件（STRUCTURE格式）：包含3764个SNP的数据集，第二列包含种群信息。对应文件：MainDataSetSTRUCTUREfile.txt
6. 非中性SNP文件（STRUCTURE格式）：基于Bayescan软件检测得到的非中性SNP位点结果，STRUCTURE文件中包含种群ID。对应文件：NonNeutralSNPstructure.txt
7. 环境数据：用于种群相关性分析的种群环境数据集。对应文件：EnvirodatagrandTerreOvAlldatapops.csv
8. 最大SNP数据集统计结果（基于STACKS软件）：参数设置为-m 8 –N 2 –M 4 –n 3 -t，本研究论文中使用了该数据集的过滤版本，该数据集要求SNP在2个种群中出现比例达90%方可被判定。对应归档文件：Archive 2.zip
9. STACKS数据集统计结果（数据集2）：参数设置为-m 8 –N 2 –M 4 –n 3 -t，该数据集要求SNP在所有种群中均有覆盖度方可被判定。对应归档文件：Archive.zip
10. 同域种群STRUCTURE格式文件：针对同域种群的STRUCTURE格式数据集文件。对应文件：structureSYMPATRICpopNOSINGLESp2r5sym.txt
11. 同域数据集STACKS统计结果：同域数据集的原始STACKS统计结果。对应归档文件：Archive.zip
12. STACKS数据集统计结果（数据集3）：针对SNP数据集3的原始统计结果（参数设置为-m 8 –N 2 –M 4 –n 3 -t），该数据集要求SNP在3个种群中90%的个体内出现方可被判定。对应归档文件：Archive.zip
13. 索引E第1部分原始RAD测序读段（RAD reads）：原始RAD测序读段，条码与编码信息可在电子表格中查询。对应归档文件：Archive.zip
14. 索引E第2部分原始RAD测序读段：原始RAD测序读段，个体详细信息请参考电子表格，为已完成测序解复用的索引E第2部分数据。对应归档文件：Archive 2.zip
15. 索引F第1部分原始RAD测序读段：原始RAD测序读段，编码信息与电子表格对应。对应归档文件：Archive.zip
16. 索引F第2部分原始RAD测序读段：原始RAD测序读段，编码与条码信息均在电子表格中。对应归档文件：Archive 2.zip
17. 索引G第1部分原始RAD测序读段：原始RAD测序读段，编码信息见电子表格。对应归档文件：Archive.zip
18. 索引G第2部分原始RAD测序读段：原始RAD测序读段，编码信息见电子表格。对应归档文件：Archive 2.zip
19. 索引H第1部分原始RAD测序读段：原始RAD测序读段，编码信息见电子表格。对应归档文件：Archive.zip
20. 索引H第2部分原始RAD测序读段：原始RAD测序读段，编码信息见电子表格。对应归档文件：Archive 2.zip
21. 索引I第1部分原始RAD测序读段：原始RAD测序读段，编码信息见电子表格。对应归档文件：Archive.zip
22. 索引I第2部分原始RAD测序读段：原始RAD测序读段，编码信息见电子表格。对应归档文件：Archive 2.zip
23. 基因组DNA正向测序读段：用于组装布纹蜗牛属部分线粒体基因组的正向读段，样本采自Mépouiri，测序平台为HiSeq。对应文件：120223_I238_FCC0HH4ACXX_L5_SZAXPI005962-142_1.fq.gz
24. 基因组DNA反向测序读段：布纹蜗牛属基因组DNA的反向测序读段。对应文件：120223_I238_FCC0HH4ACXX_L5_SZAXPI005962-142_2.trimmed.paired.fq.gz
25. ND2基因RAxML系统发育树文件：基于ND2基因构建的RAxML系统发育树原始文件。
26. 布纹蜗牛属形态测量数据（优化说明版）：文件内附详细注释说明，与其他形态测量数据文件包含相同数据，但注释更为详尽，同时包含原始坐标及普氏距离（Procrustes Distance）坐标。对应文件：PlacostylusMorphometricData.xlsx