Proteome raw data for substrate search of the cysteine protease T6SS effector Cpe1

This dataset contains raw mass spectrometry data generated in the course of studying the proteolytic effector Cpe1 and its substrates. Specifically, it includes: Proteome-wide analysis of Cpe1Bpa-crosslinked complexes using LC-MS/MS, aimed at identifying proteins in close proximity to or interacting with Cpe1. MALDI-TOF results showing the cleavage fragments of GyrB and ParE after in vitro treatment with Cpe1, supporting substrate identification and cleavage site mapping. LC-MS/MS analysis of Cpe1-cleaved GyrB and ParE fragments, providing high-resolution data for peptide identification and further confirmation of Cpe1’s proteolytic activity and specificity. All data were generated to support the biochemical characterization of Cpe1’s substrate profile and cleavage mechanism. The files are provided in their raw formats for transparency and reanalysis.本数据集包含研究蛋白水解效应因子Cpe1及其底物过程中产生的原始质谱数据。具体涵盖以下内容： 采用液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry，LC-MS/MS）对Cpe1Bpa交联复合物开展全蛋白质组分析，旨在鉴定与Cpe1紧密邻近或存在相互作用的蛋白质。 基质辅助激光解吸电离飞行时间质谱（Matrix-Assisted Laser Desorption/Ionization Time of Flight，MALDI-TOF）结果展示经Cpe1体外酶解处理后GyrB与ParE的酶切片段，可为底物鉴定与酶切位点定位提供支持。 针对Cpe1酶解产生的GyrB与ParE片段的LC-MS/MS分析，可为肽段鉴定提供高分辨率数据，并进一步验证Cpe1的蛋白水解活性与底物特异性。 所有数据均为支撑Cpe1底物谱与酶切机制的生化表征研究所生成，所有文件均以原始格式提供，以保障研究透明度并便于后续重新分析。