Specific COX-2 inhibitor confers complete protection against both PAF-induced lethality and delayed death due to PAF and hyperactivated TLR-4.

Mice were divided into 7 groups containing 6 animals each. Three groups received an intraperitoneal injection of NS-398 (20 mg/kg body wt) 30 min before receiving PAF, LPS, or a combination of both. NS-398 (20 mg/kg body wt) completely abolished PAF-induced sudden death and enhanced the cross-tolerance effect exerted by the high dose of LPS (20 mg/kg). The animals were monitored for survival for up to 6 days. The results are representative of 3 individual trials.将小鼠分为7组，每组各6只。其中3组在给予血小板活化因子（PAF）、脂多糖（LPS）或二者联合处理前30分钟，腹腔注射NS-398（20 mg/kg体重）。NS-398（20 mg/kg体重）可完全阻断PAF诱导的猝死，并增强了高剂量LPS（20 mg/kg）所介导的交叉耐受效应。对所有动物开展最长6天的存活状态监测，本实验结果可代表3次独立重复试验的结论。