CIL:12376, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library

Time series showing the dynamics of kinetchore microtubules during meiosis II in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed using 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the time series is included in this grouped set.

本时间序列展示了经人工扁平化处理的饰纽大蚊（Nephrotoma suturalis）初级精母细胞减数分裂II期间动粒微管（kinetochore microtubules）的动态变化过程。实验采用尼康（Nikon）Microphot SA显微镜搭载液晶偏振光显微镜系统（LC-PolScope, CRi, 马萨诸塞州沃本市），配备60x/1.4 PlanApo油浸物镜、1.4数值孔径油浸聚光镜及2.0倍变焦镜头，开展延时偏振光显微成像。成像过程中使用QImaging Retigo EXi型CCD相机，每15秒采集一张图像。原始图像采用5帧算法（Shribak与Oldenbourg，2003年）进行后期处理。本数据集组中同时包含该时间序列对应的动态影像。