CIL: 50760, Drosophila melanogaster. In Cell Image Library

Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective

成像模式：差分干涉对比显微术（DICM）；可视化方法：转盘共聚焦DICM与宽视场DICM。本实验采用两种成像配置：其一为搭载40×、0.95数值孔径物镜及滨松（Hamamatsu）ORCA-Flash 4.0 V3数字CMOS相机的尼康（Nikon）TiE显微镜；其二为搭载横河（Yokogawa）CSU-X1扫描单元、Andor iXon 888电子倍增电荷耦合器件（EMCCD）相机，以及60×、1.3数值孔径水浸物镜或40×、0.95数值孔径物镜的尼康（Nikon）TiE转盘共聚焦显微镜。