Reductase activity of hPDI and its mutants.

The mutants were designed to interfere with inter-domain interactions. The activity was determined according to insulin reduction as described in the text. The activity of WT PDI was taken as 100%. Date were expressed as mean ± S.D. (n = 3). Statistical significance was analyzed by using two-tailed t-test, *** and * represent P本研究所设计的突变体旨在干扰结构域间的相互作用。PDI的酶活性按照本文所述的胰岛素还原法进行测定。将野生型PDI（WT PDI）的活性设为100%。实验数据以平均值±标准偏差（S.D.）表示，n=3。统计学显著性采用双侧t检验进行分析，其中***与*分别代表P值。