Abid lab research data for article named: Optimization of mito-roGFP protocol to measure mitochondrial oxidative status in human coronary artery endothelial cells

This dataset provides useful information for researchers planning to standardize the mito-roGFP protocol in their labs. To better understand this data, we suggest using the notebook notes as a guide to visualize the original .nd2 file and excel graph report. Alternatively, a summary of graph reports for each day and sample is provided as a .pptx file and can be visualized along with the notebook notes for a clear understanding of hydrogen peroxide optimization procedures. This data was obtained from a culture of HCAEC at passage 3. Note: .nd2 files can be visualized with the free version of NIS Elements software.

本数据集可为计划在实验室中标准化线粒体-roGFP（mito-roGFP）实验方案的研究人员提供实用参考信息。为便于更好地理解本数据集内容，建议以实验记录本笔记作为指引，对原始.nd2文件与Excel图表报告进行可视化处理。此外，本数据集还提供了以.pptx格式存储的每日与样本图表报告汇总文件，可配合实验记录本笔记一同查看，以清晰理解过氧化氢优化实验流程。本数据集的数据取自第3代人冠状动脉内皮细胞（HCAEC）培养物。注：.nd2文件可通过NIS Elements软件的免费版本进行可视化查看。