LMAS Supplemental Material

LMAS is an automated workflow enabling the benchmarking of traditional and metagenomic prokaryotic de novo assembly software using defined mock communities. As proof of concept, the eight bacterial genomes and four plasmids, in tripled form, of the ZymoBIOMICS Microbial Community Standards are made available to be used as reference. Simulated raw sequence data of the mock communities with evenly distributed reads, generated from the reference genomes, with and without error, were obtained with inSilicoSeq. Project repository: https://github.com/cimendes/LMASLMAS是一款自动化工作流，可基于定义明确的模拟群落（mock communities）对传统及宏基因组原核生物从头组装（de novo assembly）软件进行基准评测。作为概念验证研究，本研究采用ZymoBIOMICS微生物群落标准品（ZymoBIOMICS Microbial Community Standards）中的8个细菌基因组与4个质粒，并以三重重复形式提供，可作为参考序列使用。基于上述参考基因组，借助inSilicoSeq工具生成了测序读段均匀分布的模拟群落原始测序数据，涵盖存在测序错误与无测序错误两种场景。本项目的代码仓库地址为：https://github.com/cimendes/LMAS